integrin αvβ3  (Bioss)

Journal: Science Advances

Article Title: Dynamic adaptive coassembled sericin protein orchestrating stem cell development for nucleus pulposus regeneration

doi: 10.1126/sciadv.adx2768

Figure Lengend Snippet: ( A ) The composition and structure of the NP in IVD tissue exhibited spatial heterogeneity, which support NP cell development and physiological function through dynamic mechanical regulation and biochemical signaling. ( B ) Inspired by the partitioned structure of ECM, bioactive sericin was coassembled with BPAA-GFF fibrillar networks, driven by phase separation–induced molecular network rearrangement. The resulting dynamic adaptive network activated stem cell integrin ligands to modulate cell-matrix interactions and promoted chondrogenic differentiation via rapid stress relaxation and biochemical factor induction. ( C ) In rat and rabbit IVD degeneration models, KGN-incorporated coassembled hydrogel effectively promoted BMSCs for NP regeneration.

Article Snippet: To investigate the role of integrin β3 in mediating stem cell adhesion and cytoskeletal regulation, functional inhibition was performed using the αvβ3 integrin antagonist cilengitide (MedChemExpress, USA).

Techniques:

Journal: Science Advances

Article Title: Dynamic adaptive coassembled sericin protein orchestrating stem cell development for nucleus pulposus regeneration

doi: 10.1126/sciadv.adx2768

Figure Lengend Snippet: ( A ) 3D coculture of stem cells with the coassembled BS gel. ( B ) Time-dependent gene expression trend of stem cells regulated by coassembled BS protein gel. ( C ) Up-regulated genes and their associated biological events based on Gene Ontology (GO) analysis comparing BS versus B. ( D ) Flow relationships between activated molecular pathways and up-regulated genes based on Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis comparing BS versus B. JAK-STAT, Janus kinase–signal transducer and activator of transcription; MAPK, mitogen-activated protein kinase. ( E ) Gene interaction network analysis and Gantt chart analysis of temporal gene expression in three gene categories. ( F ) Gene expression heatmap analysis comparing BS versus B, n = 3 independent samples. ( G ) In vitro validation of gene expression by qPCR, n = 3 independent samples. ( H ) Local interaction residues in molecular docking. Color-coded representation shows BPAA-GFF (green), sericin protein (magenta), and the integrin αvβ3 complex (blue and orange). ( I ) Time-course analysis of stem cells cocultured with the hydrogel at 1, 2, and 4 weeks. ( J ) Integrin β3 expression analysis at 1 week, n = 3 independent samples. ( K ) Cytoskeletal staining and cell aspect ratio analysis at 1 week. ( L ) YAP staining and nuclear/cytoplasmic ratio analysis. ( M ) Coassembled BS gel activates integrin-mediated cell-matrix interactions, driving YAP nuclear translocation and downstream mechanotransduction signaling. Statistical analyses were performed with Student’s t tests (unpaired and two-tailed): * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: To investigate the role of integrin β3 in mediating stem cell adhesion and cytoskeletal regulation, functional inhibition was performed using the αvβ3 integrin antagonist cilengitide (MedChemExpress, USA).

Techniques: Gene Expression, In Vitro, Biomarker Discovery, Expressing, Staining, Translocation Assay, Two Tailed Test

Journal: International Journal of Molecular Sciences

Article Title: Peptide-Guided Photodynamic Therapy via Integrin αvβ6 in Pancreatic Cancer

doi: 10.3390/ijms27041838

Figure Lengend Snippet: ( a ) Expression of αvβ6 and αvβ3 integrins in pancreatic cancer cell lines. ( a ) Normalized fluorescence measurements showing integrin expression levels in BxPc3, MiaPaca2, and Panc1 cells. BxPc3 cells display significantly higher αvβ6 expression compared with MiaPaca2 and Panc1, whereas αvβ3 expression is detectable at lower levels with a different expression profile across cell lines. Statistical analysis was performed using one-way analysis of variance (ANOVA) followed by post hoc multiple comparison testing; differences were considered statistically significant at *** p < 0.001 (* p < 0.05 where indicated). ( b ) Western blot results showing β6 integrin expression levels normalized to protein concentration (pg β/µg protein) across different PDAC cell lines. BxPc3 expresses high levels of β6 (1411 pg/µg), MiaPaca2 and Panc1 expresses show no detectable levels of this integrin.

Article Snippet: Primary antibodies included Anti-Integrin αvβ3 (Integrin β3 (D7X3P) XP ® Rabbit mAb, Cell Signaling) at a 1:500 dilution and Anti-Integrin αvβ6 (clone EM05201, ZooMAb ® Rabbit Monoclonal ZRB1104) at a 1:1000 dilution, incubated overnight at 4 °C.

Techniques: Expressing, Fluorescence, Comparison, Western Blot, Protein Concentration